Tag Archives: DNA synthesis

BioXp™ Genomic Workstation Makes European Debut at VIB Research Institute in Belgium

SGI-DNA’s benchtop automated DNA printer to accelerate genomic research as part of VIB’s Technology Watch Initiative 

SGI-DNA Inc., a Synthetic Genomics Inc. (SGI) company, and VIB, a Belgian life science research institute, today announced that VIB will become the first institute in Europe to integrate the BioXp™ 3200 System in its workflows as part of its vision for adopting breakthrough technologies.  The BioXp™ 3200 System is the world’s first benchtop automated genomic workstation that rapidly prints high-quality, double-stranded DNA fragments and clones into any vector in an overnight run. With the BioXp™ 3200 System, VIB will be accelerating turnaround time for generating custom DNA for genomic research.

VIB’s Technology Watch Initiative implements new, emerging, and disruptive technologies into its laboratories and research facilities. Active since 2008, the Technology Watch program evaluates strategic technology investment opportunities and provides the funding to secure prototype instruments and early-stage technologies from high-potential fields such as Synthetic Biology, CRISPR, and single-cell analysis. The BioXp™ 3200 System will be placed in the lab of Thomas Jacobs, Ph.D., a VIB scientist in the VIB-UGent Center for Plant Systems Biology,  who is developing and optimizing plant genome editing through the use of CRISPR/Cas systems.

“The BioXp™ 3200 System will fundamentally change the way our research group approaches cloning. Not only will it greatly reduce our turnaround time for cloning projects, but also will hopefully open up new ways to test the gene editing systems we are developing,” says Dr. Jacobs.

“By automating rapid DNA synthesis, this will put VIB in a competitive position, allowing researchers to focus their time on the crucial scientific questions rather than on routine lab work,” adds Halina Novak, Ph.D., Technology Watch program manager at VIB.

Scientists are able to specifically design and clone genes with the BioXp™ System to answer key questions in their function  in biological systems. With the BioXp™ 3200 System, researchers do not need a template to obtain a gene of interest. Genes are built in silico, designed with sequence modifications to address the specific research.

“Automating gene synthesis promotes scientific discovery and advancement,” says Nathan Wood, president of SGI-DNA. “The global expansion of the BioXp™ 3200 System, notably to one of Europe’s most innovative research institutes, will help to facilitate further genomic breakthroughs in life science research.”

More information is available at Sgidna

Source: VIB
Picture: ©VIB-Ine Dehandschutter

BioXp

BioXp3200

The BioXp DNA printer from Synthetic Genomics, Inc. allows you to obtain high quality DNA in as fast as 1.5 weeks at competitive pricing. These so-called DNA-tiles (max 32 in parallel) can be automatically cloned into the companies provided pUCGA 1.0 vector or your custom vector of interest during the same run.

How it works?

You submit your sequence through the order portal of SGI-DNA, after which you receive an oligo plate some days later. This is, along with a reagents plate, placed in the BioXp DNA printer. After closing of the lid, the instrument verifies automatically if all the required components are present: the oligo plate, a reagents plate, a recovery plate, an ethanol reservoir, 50 and 200 µl tips and the purification strip. Once this check is finished, a simple click on the touchscreen will allow you to start the run and your DNA will be synthesized while you are sleeping. After the overnight run, your DNA fragments or cloned vectors can be found back in the recovery plate.

Evaluation within VIB?

VIB was the first academic institute in Europe acquiring the BioXp3200 and the instrument was evaluated as part of the VIB Tech Watch ‘Flexible Innovation Lab’. The technology was first adopted by Thomas Jacobs, expert scientist at the VIB Center of Plants Systems Biology (PSB) with a passion for synthetic biology. A smoothly running weekly DNA synthesis service was set-up, with big interest from multiple PSB groups. The DNA synthesis service was afterwards opened to all VIB scientists by Silvie Van den Hoecke, Life Science Technology Specialist at VIB Tech Watch. Orders were received from multiple groups across the different VIB departments. The quality of the synthesized DNA was validated on agarose gel electrophoresis and the error rate inquired. After a positive evaluation in the first five months, it was decided to integrate the BioXp3200 DNA printer within VIB, and a weekly DNA synthesis service will be run from within PSB.