Oxford Nanopore Technologies (ONT) sequencing uses biological nanopores embedded into a lipid membrane to sequence DNA. While DNA is passing through the pores, subtle structural rearrangements are induced in the pore structure. These rearrangements are sequence specific and, as such, can be used to determine the DNA sequence afterwards. The library preparation for this sequencing technology is limited, since only a motor protein should be added, which unwinds the DNA and enables is to pass as ssDNA through the pores. This is a huge advantage compared to other NGS technologies, which require DNA fragmentation, adaptor ligation, size selection and amplification prior to sequencing, which might induce amplification bias.